Uncut Sheet

Lateral flow tests are paper-based rapid diagnostic tests for qualitatively detecting the presence, absence, or concentration of analytes of interest in specimens. Apart from the type of buffer used, a lateral flow test consists of a cassette and a strip. After the sample is mixed with the buffer, the flow takes place on the strip. A strip is an uncut sheet cut to the appropriate size for the test. Starting from where the buffer-sample mixture is placed, the strip consists of four parts: sample pad, conjugate pad, flowing pad, and absorbent pad.

The sample pad is the first contact with the sample. The sample pad ensures and maintains a continuous flow of the liquid sample. In addition, it is also used to accumulate dry reagents that provide the appropriate pH adjustment for the test being performed. Next, the flowing sample reaches the conjugate pad. The conjugate pad is used for labeling the analytes in the sample. This label ensures that the target analytes are signaled in the test and control section. Thus, the presence or absence of the targeted analyte can be detected. Next, the sample passing through the conjugate pad enters the flowing membrane (nitrocellulose membrane). The flowing membrane is where the test and control area binds with the immobilized protein to capture the analytes. Its porous structure ensures a consistent flow of the sample, maintaining the stability of the protein against the non-specific binding. In addition, the capillary flow rate of the membrane affects the run time of the assay because the sample flow rate determines the time between labeling and capture probes in the test and control sections. Finally, the sample reaches the absorbent pad. The purpose of the absorbent pad is to absorb excess samples and prevent backflow, thus avoiding false positives. All these parts combine to form the strip. The length, width, types of materials to be used in the pads, and length of the pads vary according to the test. The uncut sheet is the state of the strips before they are cut to the desired length and width. Pads are placed on the uncut sheet.

As 1sensDX, we are at your service with our uncut sheet manufacturing. With our expert and experienced team, we are at your side in the production of uncut sheets. As a result, you can produce high-quality, sensitive, and reliable rapid diagnostic tests with our uncut sheets suitable for the desired rapid diagnostic test.

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Colloidal Gold

Colloidal gold is also among the most widely used and favored markers for detecting antibodies and antigens in lateral flow assays and electron microscopy.

Conjugation Kits

A reporting system is needed to detect or measure the presence of a biological marker. For example, the first virologic tests allowed viral antigens to bind to visible targets such as erythrocytes.

Uncut Sheet

Lateral flow tests are paper-based rapid diagnostic tests for qualitatively detecting the presence, absence, or concentration of analytes of interest in specimens.