Conjugation Kits

A reporting system is needed to detect or measure the presence of a biological marker. For example, the first virologic tests allowed viral antigens to bind to visible targets such as erythrocytes. Although some such techniques were developed, they were only effective if sufficient antigen or antibody was present, and results could be misinterpreted due to their low sensitivity or specificity. For these reasons, more sensitive and robust detection systems have been developed those bind proteins to the new marker and measure the rate of change with more accurate and reliable instruments.

The word conjugation is originally derived from a Latin word meaning “to bind together.” A protein to which another chemical group or molecule is attached is called a conjugated protein. Covalent bond interactions are typically used to conjugate molecules to proteins. There are many different methods of protein conjugation. These include radioactive isotopes, fluorophores, and enzymes such as Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Another name for these enzymes is detection enzymes. Covalent labeling and conjugation of proteins are seen as crucial steps for many types of biological experiments to take place. This process makes obtaining the desired data more accessible and increases the results’ sensitivity. Commercial kits, reagents, and services are available that offer many options to simplify the protein conjugation process.

Antibody conjugation, also known as antibody labeling, is the process of chemically linking a specific label to an antibody. They require a certain level of skill and expertise and have complex protocols. But despite all this, there are several standard methods for conjugating antibodies. Due to their high specificity, antibodies are widely used to detect and quantify antigens in numerous applications such as flow cytometry, ELISA, western blotting, immunohistochemistry and lateral flow. Primary antibody is the name given to the antibody that binds to the antigen.

Conventional conjugation methods require a degree of expertise and hands-on time on the bench. However, the development of conjugation kits has simplified the process while providing flexibility during experiment design. These kits contain every component needed for conjugation. There is little room for error, minimizing antibody loss. The wide range of labels and kit sizes provides flexibility and scalability. The simple procedure saves time in the lab and speeds up the transition to other steps.

We offer a robust portfolio of different conjugation kits for screening, optimizing, and developing lateral flow assays. Our conjugation kits ensure time and resource efficiency throughout research and development by providing a quick and simple method to screen, validate, and label various biomolecules, including antibodies, proteins, peptides, and enzymes. Moreover, by removing pre-activation requirements and minimizing hands-on time, our one-step conjugation kits accelerate the entire procedure of conjugate generation.

Altogether, our conjugation kits contain every component required for the screening, validating, and labeling of the reagents and supplies of your choice. Whether you seek to improve existing assays or develop new lateral flow assays, our kits are ideal for screening and optimizing reagents and supplies before the onset of scale-up production.

ALL PRODUCT

Colloidal Gold

Colloidal gold is also among the most widely used and favored markers for detecting antibodies and antigens in lateral flow assays and electron microscopy.

Conjugation Kits

A reporting system is needed to detect or measure the presence of a biological marker. For example, the first virologic tests allowed viral antigens to bind to visible targets such as erythrocytes.

Uncut Sheet

Lateral flow tests are paper-based rapid diagnostic tests for qualitatively detecting the presence, absence, or concentration of analytes of interest in specimens.